APPLIED PLANT BIOLOGY (PLANT BIOTECHNOLOGY)

(Text with Practical) – For Semester VI (NEP–FYUGP)
B.Sc. Botany

Authors: Mr. Bhaskor Kolita & Dr. Rubul Saikia
Publisher: Mahaveer Publications

? About the Book

Applied Plant Biology (Plant Biotechnology) is a comprehensive Semester VI (NEP–FYUGP) textbook specially designed for B.Sc. Botany students of:

• Gauhati University

• Jagannath Barooah University

• Assam University

• Bodoland University

• North Lakhimpur University

This book provides a clear, systematic and student-friendly approach to Plant Biotechnology, covering both theoretical concepts and practical applications as prescribed in the latest NEP syllabus.

? Key Features

✔️ Written strictly as per FYUGP–NEP syllabus
✔️ Covers both Theory and Practical components
✔️ Simple and exam-oriented explanation of complex topics
✔️ Well-structured chapters for better conceptual clarity
✔️ Includes modern biotechnological techniques and applications
✔️ Practical experiments with step-wise understanding
✔️ Suitable for university examinations and competitive preparation

? Theory Contents Overview

? Unit 1: Biotechnology & Plant Tissue Culture

• Introduction, Aim & Scope of Biotechnology

• History and Fundamentals of Plant Tissue Culture

• Culture Media & Techniques

• Totipotency, Organogenesis & Embryogenesis

• Protoplast Isolation, Culture & Fusion

? Unit 2: Applications of Tissue Culture

• Micropropagation

• Androgenesis

• Virus Elimination

• Secondary Metabolite Production

• Haploids, Triploids & Hybrids

• Cryopreservation & Germplasm Conservation

• Advantages & Limitations

? Unit 3: Recombinant DNA Technology

• Genetic Engineering Tools & Techniques

• Restriction Enzymes & Mapping

• Cloning Vectors (pBR322, pUC18/19, Ti plasmid, BACs, YAC, Cosmids, Lambda Phage etc.)

? Unit 4: Gene Cloning

• Recombinant DNA

• Bacterial Transformation

• PCR-mediated Gene Cloning

• Genomic & cDNA Libraries

• Screening Techniques

? Unit 5: Methods of Gene Transfer

• Agrobacterium-mediated Transfer

• Electroporation

• Microinjection

• Microprojectile Bombardment

• Selection & Screening of Transgenics

? Unit 6: Applications of Biotechnology

• Agricultural Biotechnology

• GMOs & Transgenic Plants

• Bt Cotton, Golden Rice, Flavr Savr Tomato

• Bioremediation

• Edible Vaccines

• Industrial Enzymes

• Biosafety Concerns

? Practical Section Includes

• Preparation of MS Medium

• In vitro Sterilization & Inoculation

• Anther, Embryo & Endosperm Culture

• Protoplast Isolation

• Restriction Mapping

• Gene Transfer Techniques (Photographic Study)

• Isolation of Plasmid DNA

• Restriction Digestion & Gel Electrophoresis

? Who Should Buy This Book?

• B.Sc. Botany Semester VI students (FYUGP – NEP)

• Students preparing for MSc Entrance

• Biotechnology aspirants

• University practical examination candidates

This book serves as a complete academic resource for mastering Plant Biotechnology, integrating theoretical depth with laboratory exposure, making it highly useful for both university examinations and advanced studies.