Cell Biology

Description

“Cell Biology with Practical” by Dr. Tripurari Mishra and Dr. Diwakar Mishra is an essential textbook for B.Sc. Zoology majors, tailored for the 3rd Semester (DU), 2nd Semester (MU, NU, AU), and 6th Semester (GU) syllabi. This book offers a comprehensive exploration of cellular biology, covering the structure and function of prokaryotic and eukaryotic cells, plasma membranes, organelles like mitochondria and the endoplasmic reticulum, and the cytoskeleton. It delves into cell division, cell signaling, and apoptosis. The practical section provides hands-on exercises, including the preparation of stained slides and the study of mitosis and meiosis, making it an indispensable resource for students.

1. Introduction of Cell : Prokaryotic cell, Eukaryotic cells, Virus
Mycoplasma, Viroids, Prions
2. Plasma Membrance : Various model of Plasma Membrane, Active
and Passive Transport, Facilitated Transport Cell Junctions, Tight Junctions, Desmosomes, Gap Junction.
3. Endoplasmic Reticulum : Structure and Function of E.R., Golgi
Apparatus, Lysosomes, Endomembrane System.
4. Mitochondria : Structure of Mitochondria, Respiratory Chain,
Semiautonomous Nature, Endosymbiotic Hypothesis, Chemi-osmotic Hypothesis,
Peroxisome.
5. Cytoskeleton : Structure and Function of Microtubules, Microfilaments, Intermediate Filaments
6. Nucleus : Structure of Nucleus, Nuclear Envelope, Nuclear Pore Complext, Nucleolus Chromatic
Euchromatin and Heterochromatin and Packaging, Giant Chromosomes, Types of Eukaryotic Chromosomes based on
Centromere, DNA (Nucleosome Model)

7. Cell Division : Mitosis, Meosis, Cell Cycle and its Regulation, Cell culture, Abnormal Cell Divisions (Cancer
cells).
8. Cell Signalling : Cell Signalling, GPCR and Role of cAMP, Types of Cell Signalling Molecules, Mechanisms of
Signal Transductions of Peptides and Steroid Hormone.
9. Cell Death  : Necrosis and Apoptosis, Significance of Apoptosis in Cellular Homeosfasis, Mechanism
of Apoptosis.
10. Practical : Preparation of temporary stained squash of onion root tip to study various stages of
mitosis.
• Study of various stages of meiosis.
• Preparation of permanent slide to show the presence of Barr body in human female blood cells/cheek cells.
• Preparation of permanent slide to demonstrate :
(i) DNA by Feulgen Reaction (ii) Mucopolysaccharides by PAS Reaction
(iii) Proteins by Mercuro Bromophenol Blue/fast Green
(iv) Lipid by Sudan Black-B.
References