Plant Biotechnology (With Practical) by Dr. Swapnil Yadav, published by Mahaveer Publications, is a comprehensive and student-friendly textbook written in accordance with the FYUGP–NEP syllabus for B.Sc. 6th Semester Botany Honours students of Dibrugarh University, Gauhati University, and other Indian universities.

The book provides a detailed introduction to the principles, methods, and applications of plant biotechnology, integrating both theoretical and practical aspects essential for undergraduate learners. It covers fundamental topics such as plant tissue culture, genetic engineering, transgenic plants, molecular markers, biofertilizers, biopesticides, and biotechnological applications in agriculture and industry.

Special emphasis is placed on plant tissue culture techniques—including micropropagation, somatic embryogenesis, protoplast fusion, and cryopreservation—which form the backbone of modern plant biotechnology. The book also discusses recent advancements in genetic transformation methods, molecular diagnostics, and sustainable biotechnology practices relevant to global and Indian contexts.

The practical section provides step-by-step laboratory procedures, equipment handling techniques, and experimental exercises designed to strengthen students’ hands-on skills in plant biotechnology.

Authored by Dr. Swapnil Yadav, this textbook combines clarity, academic depth, and modern scientific perspectives, making it ideal for undergraduate students, educators, and competitive exam aspirants in the field of life sciences and biotechnology.

Key Features:

• Based on the FYUGP–NEP syllabus for B.Sc. 6th Semester Botany Honours.

• Comprehensive coverage of plant tissue culture and genetic engineering.

• Includes recent developments in plant biotechnology and molecular biology.

• Step-by-step practical exercises and experiments with illustrations.

• Clear explanations supported by diagrams, charts, and examples.

• Written in simple, lucid, and examination-oriented language.

• Ideal for students of Botany, Biotechnology, Agriculture, and Life Sciences.

• Published by Mahaveer Publications, a reputed name in academic publishing.

  COURSE CONTENTS

  Unit 1: Introduction

  • Plant Tissue Culture

  • Recombinant DNA Technology

  • Fermentation

  • Waste Treatment

  • Biofuel

  • Biofertilizer

  • Scope of Biotechnology

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  Unit 2: Plant Tissue Culture

  • History of Plant Tissue Culture

  • General Techniques

  • Micropropagation

  • Advantages

  • Limitations

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  Unit 3: Tissue Culture Media

  • Constituents of Media

  • Types of Media

  • Preparation and Sterilization of Media

  • Role of Growth Regulators

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  Unit 4: Totipotency, Organogenesis, and Embryogenesis

  • Concept of Totipotency

  • Organogenesis and Somatic Embryogenesis

  • Types of Organogenesis and Somatic Embryogenesis

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  Unit 5: Protoplast Culture

  • Isolation of Protoplast

  • Purification of Protoplast

  • Viability Testing of Protoplast

  • Culture of Protoplast

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  Unit 6: Somatic Hybridization

  • Methods of Protoplast Fusion

  • Identification and Selection of Hybrid Cells

  • Cybrids

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  Unit 7: Haploid Culture

  • Anther Culture

  • Pollen Culture

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  Unit 8: Cryopreservation

  • Principles and Methods

  • Applications in Germplasm Conservation

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  Unit 9: Secondary Metabolite Production

  • Biosynthesis of Secondary Metabolites

  • Microbial and Plant Cell Cultures for Secondary Metabolite Production

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  Unit 10: Meristem Culture

  • Development of Virus-Free Plants

  • Clonal Propagation

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  Unit 11: Recombinant DNA Technology

  • Steps and Tools of rDNA Technology

  • Applications and Limitations

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  Unit 12: Preparation and Separation of DNA Fragments

  • Restriction Enzymes

  • Joining of DNA Fragments (Linkers, Adaptors)

  • Restriction Maps

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  Unit 13: Vectors

  • Plasmids

  • PBR322 and pUC

  • Bacteriophage

  • Cosmids

  • Phagemids

  • Ti Plasmid

  • Artificial Chromosomes

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  Unit 14: Polymerase Chain Reaction (PCR)

  • Principle and Technique

  • Applications of PCR in Biotechnology

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  Unit 15: DNA Libraries

  • Genomic Library

  • cDNA Library

  • Colony Hybridization Test

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  Unit 16: Methods of Gene Transfer

  • Agrobacterium-Mediated Gene Transfer

  • Electroporation

  • Biolistics (Gene Gun)

  • Microinjection

  • Liposome-Mediated Gene Transfer

  • Marker Genes and Reporter Genes

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  Unit 17: Applications of Biotechnology

  • Production of Transgenic Plants:

    • Roundup Ready Soybean

    • FLAVR SAVR Tomato

    • Golden Rice

    • Human Insulin

    • MoonDust Carnations

  • Bioremediation (Superbug)

  • Industrial Enzyme Production

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  PRACTICALS

  1. Preparation of Plant Tissue Culture Media.

  2. Sterilization Techniques for Media and Instruments.

  3. Demonstration of Callus and Organ Culture.

  4. Study of Somatic Embryogenesis and Micropropagation.

  5. Observation of Protoplast Isolation Techniques.

  6. Demonstration of DNA Isolation and Gel Electrophoresis.

  7. Study of PCR and its Applications.

  8. Preparation of Biofertilizer Samples and their Analysis.

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