? Plant Biochemistry & Molecular Biology

Author: Dr. Kalyan Das
Published by: Mahaveer Publications, Dibrugarh
Applicable for: Dibrugarh University & Other Indian Universities
Course Type: Core / Major – IV (FYUGP NEP Syllabus, Semester III – Botany)

? Course Overview:

This textbook provides a comprehensive and systematic insight into Plant Biochemistry and Molecular Biology, specially tailored for Semester-III Botany Major students as per the FYUGP NEP and CBCS syllabus of Dibrugarh University and other Indian universities. Authored by Dr. Kalyan Das, this book is structured to offer theoretical depth along with practical exposure, preparing students to understand plant biomolecular functions at the molecular level.

? Course Units Covered:

UNIT 1: Introduction
Basic principles of biochemical systems and plant molecular biology.

UNIT 2: Biomolecules

• Chemical bonds, water, pH, and buffer systems.

• Carbohydrates: Monosaccharides to polysaccharides.

• Lipids: Structural & storage types, fatty acids, phosphoglycerides.

• Proteins: Structure (primary to quaternary), denaturation, and biological functions.

• Nucleic Acids: DNA & RNA structures, A, B, Z DNA forms, and tRNA.

UNIT 3: Bioenergetics

• Laws of thermodynamics.

• Free energy, redox reactions, ATP as energy currency.

UNIT 4: Genetic Material & Its Organisation

• Experimental proofs (Griffith’s, Avery, Hershey & Chase).

• DNA organization in prokaryotes, viruses, and eukaryotes.

• Organelle DNA: mitochondria and chloroplast.

UNIT 5: Genetic Code

• Structure of genetic code, protein synthesis.

• Ribosome function, tRNA charging, and post-translational modifications.

UNIT 6: Plant Hormone Biochemistry

• Biochemical mechanisms of hormone synthesis and signaling.

UNIT 7: Biochemical Tools & Techniques

• Enzyme structure and functions.

• Enzyme kinetics, Michaelis–Menten equation.

• Lab-based techniques for protein and DNA analysis.

UNIT 8: Practicals

1. Qualitative tests for biomolecules.

2. Staining techniques (DNA & cell wall).

3. Estimation of plant proteins and sugars.

4. DNA isolation and quantification.

5. Visual study of DNA replication mechanisms (Theta, Rolling Circle).

Selected Bibliography
Curated list of academic references and suggested readings for in-depth study.

? Why Choose This Book?

• Aligned with FYUGP NEP 2020 recommendations.

• Covers both theoretical concepts and lab applications.

• Written by a renowned academic expert in the field of plant sciences.

• Suitable for both Major and Minor Botany students.

Ideal for:
Undergraduate Botany students of DU, GU, NU, and other Indian universities under NEP/CBCS curriculum.